WS10 Life History Traits

Life History Traits and Genome Structure : Life History Traits and Genome Structure Aerobiosis and G+C Content in Bacteria J.R. Lobry Université Claude Bernard - Lyon I CNRS UMR 5558 andamp; INRIA Helix FRANCE


Slide2 :


Aerobic versus Anaerobic : Aerobic versus Anaerobic You need the presence of O2 to live, you are aerobic (obligate) You need the absence of O2 to live, you are anaerobic (obligate) [...snip...]


Slide4 : Only Anaerobic Species Anaerobic and Aerobic Species


Aerobic >> Anaerobic : Aerobic andgt;andgt; Anaerobic In aerobic conditions (with O2) the oxydation of one molecule of glucose (respiration) yields 38 ATP (metabolic currency unit). Short generation times. In anaerobic conditions (without O2) the fermentation of one molecule of glucose yields 2 ATP (metabolic currency unit). Long generation times. The generation time is a life history trait


Life History Traits and Genome Structure : Life History Traits and Genome Structure Aerobiosis and G+C Content in Bacteria J.R. Lobry Université Claude Bernard - Lyon I CNRS UMR 5558 andamp; INRIA Helix FRANCE


Slide7 : Bacteria in the Universal Tree of Life Pseudomonas Escherichia Halobacterium Methanobacterium Desulfurococcus Halococcus Methanococcus Thermoproteus Thermus Anacystis Bacillus Physarum Crithidia Tetrahymena Prorocentrum Saccharomyces Oryza Homo Drosophila Streptomyces EUCARYA ARCHAEA EUBACTERIA « BACTERIA » ?


Slide8 : Only Bacteria Bacteria and Eucarya


Bacterial Genomes:Circular dsDNA : Bacterial Genomes: Circular dsDNA Here G+C = 50% The G+C content is a genome structure


Life History Traits and Genome Structure : Life History Traits and Genome Structure Aerobiosis and G+C Content in Bacteria J.R. Lobry Université Claude Bernard - Lyon I CNRS UMR 5558 andamp; INRIA Helix FRANCE


Aerobiosis and G+C Content in Bacteria : Aerobiosis and G+C Content in Bacteria Aerobic Anaerobic Naya et al. (2002) J. Mol. Evol., 55:260


Aerobiosis and G+C Content in Bacteria : Aerobiosis and G+C Content in Bacteria Aerobic Anaerobic Naya et al. (2002) J. Mol. Evol., 55:260 Why?


Metabolic cost of amino-acids in Aerobiosis : Metabolic cost of amino-acids in Aerobiosis Akashi andamp; Gojobori (2002) PNAS, 99:3695 11.7 ATP 38.3 ATP 11.7 ATP 52.0 ATP 15.3 ATP 14.7 ATP 27.3 ATP 27.3 ATP


From amino-acid cost to Protein cost : From amino-acid cost to Protein cost Akashi andamp; Gojobori (2002) PNAS, 99:3695 Protein cost in ATP Protein concentration in cells


So what? : So what?


The growth of genomic data : The growth of genomic data But available fossil DNA is andlt; 0.00013 109 years...


Slide17 : All data are here


Dataset : Dataset ~300 bacterial species ~1,000,000 proteins ~100,000,000 amino-acids


Slide19 : Akashi (2002) Naya (2002)


Interpretation : Interpretation Aerobic bacteria have a higher genomic G+C content than anerobic bacteria because this induces an average protein composition which is less expensive in aerobic conditions


Life History Traits and Genome Structure : Aerobiosis and G+C Content in Bacteria Life History Traits and Genome Structure J.R. Lobry Université Claude Bernard - Lyon I CNRS UMR 5558 andamp; INRIA Helix FRANCE