logging in or signing up reproduction 3 Freedom Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 187 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: August 11, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Slide1: 1. Feminization 2. Masculinization 3. Triploidization 4. Cryopreservation 5. Broodstock management Slide2: Monosex culture - advantages Higher growth rate of one sex Prevention of energy diversions Reduces aggressive interactions Greater uniformity of size at harvest Avoids reduction in product quality Reduces unfavorable EI through escapees Slide3: 1. Feminization Two strategies: Hormone therapy Gynogenesis Slide4: Hormone therapy Hormonal feminization is employed by industry – salmonid culture Two methods employed: 1. Direct feminization 2. Indirect feminization Slide5: 1. Direct feminization Sexually undifferentiated fish All-female stocks Estrogen treatment Slide6: 1. Direct feminization Applicable to any species Employs estradiol-17b Treatment during early stages of differentiation Most widely used method No treatment-based mortality Public perception disadvantages Females when mated will produce 50:50 ratio Slide7: 2. Indirect feminization – female homogamety Slide8: 2. Indirect feminization – female homogamety Females must be homogametic sex Masculinization of females More than 1 generation to complete Sex specific probes allow separation of XX neomales Market fish not treated with steroids Used with chinook salmon and trout commercially Slide9: Gynogenesis Egg stimulated to divide by genetically inactivated spermatozoon Spermatozoon inactivated by UV Induced diploidization by pressure shock Slide10: Gynogenesis Low survival ~ 50% Abnormal ovaries May produce variable sex ratios Use of females for monosex production Slide11: Lability Slide12: Slide13: Oestrogen and Oncorhynchus sp. Slide14: 2. Masculinization - methods Sorting Hormonal sex reversal Interspecific hybridization Androgenesis Supermales Triploidy Transgenesis? Slide15: Interspecific hybridization Oreochromis niloticus x O. aureus O. mossambicus x O. aureus O. Mossambicus x O. hornorum O. spilurus niger x O. macrochir O. spilurus niger x O. hornorum O. aureus x O. hornorum Tilapia zillii x O. andersonii All progenies monosex Slide16: 3. Triploidization Produces sterile animals Subject eggs to an environmental shock: 1. heat 2. pressure 3. cold 4. chemical 5. electric Slide17: Slide18: 4. Cryopreservation Long-term storage of living cells using liquid nitrogen as a coolant Stored material can be maintained for centuries Important to conserve genetic material Embryo storage only reported with hard clam and oyster Slide19: Slide20: 5. Broodstock management Nutrition - vit. E; HUFA; a-tocopherol Stress Vertical transmission of diseases viral bacterial fungal You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
reproduction 3 Freedom Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 187 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: August 11, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Slide1: 1. Feminization 2. Masculinization 3. Triploidization 4. Cryopreservation 5. Broodstock management Slide2: Monosex culture - advantages Higher growth rate of one sex Prevention of energy diversions Reduces aggressive interactions Greater uniformity of size at harvest Avoids reduction in product quality Reduces unfavorable EI through escapees Slide3: 1. Feminization Two strategies: Hormone therapy Gynogenesis Slide4: Hormone therapy Hormonal feminization is employed by industry – salmonid culture Two methods employed: 1. Direct feminization 2. Indirect feminization Slide5: 1. Direct feminization Sexually undifferentiated fish All-female stocks Estrogen treatment Slide6: 1. Direct feminization Applicable to any species Employs estradiol-17b Treatment during early stages of differentiation Most widely used method No treatment-based mortality Public perception disadvantages Females when mated will produce 50:50 ratio Slide7: 2. Indirect feminization – female homogamety Slide8: 2. Indirect feminization – female homogamety Females must be homogametic sex Masculinization of females More than 1 generation to complete Sex specific probes allow separation of XX neomales Market fish not treated with steroids Used with chinook salmon and trout commercially Slide9: Gynogenesis Egg stimulated to divide by genetically inactivated spermatozoon Spermatozoon inactivated by UV Induced diploidization by pressure shock Slide10: Gynogenesis Low survival ~ 50% Abnormal ovaries May produce variable sex ratios Use of females for monosex production Slide11: Lability Slide12: Slide13: Oestrogen and Oncorhynchus sp. Slide14: 2. Masculinization - methods Sorting Hormonal sex reversal Interspecific hybridization Androgenesis Supermales Triploidy Transgenesis? Slide15: Interspecific hybridization Oreochromis niloticus x O. aureus O. mossambicus x O. aureus O. Mossambicus x O. hornorum O. spilurus niger x O. macrochir O. spilurus niger x O. hornorum O. aureus x O. hornorum Tilapia zillii x O. andersonii All progenies monosex Slide16: 3. Triploidization Produces sterile animals Subject eggs to an environmental shock: 1. heat 2. pressure 3. cold 4. chemical 5. electric Slide17: Slide18: 4. Cryopreservation Long-term storage of living cells using liquid nitrogen as a coolant Stored material can be maintained for centuries Important to conserve genetic material Embryo storage only reported with hard clam and oyster Slide19: Slide20: 5. Broodstock management Nutrition - vit. E; HUFA; a-tocopherol Stress Vertical transmission of diseases viral bacterial fungal