logging in or signing up 2b lanciotti Esteban Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 107 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: October 24, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Serological and Molecular Assays for West Nile Virus: Serological and Molecular Assays for West Nile Virus Current Techniques & ImprovementsRecommended Tests for WN Virus: Recommended Tests for WN VirusSlide3: Serological Assays for West Nile Virus human serum/csf equine serum avian (chicken) IgM ELISA IgG ELISA POS NEG PRNT with: SLE, WN, DEN STOPSlide4: WN Serological Data IgM ELISA P/N for WN > 2X IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DEN 4-fold rise in PRNT titer to WN from acute to conv. specimen Typical Human WN Case 83% of 2001 WN cases met criteriaSlide5: WN Serological Data from 2001 IgM ELISA P/N for WN < IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DENSlide6: WN Serological Data from 2001 IgM ELISA P/N for WN > 2X IgM P/N to SLE. 4-fold rise in PRNT titer to WN from acute to conv. Specimen Flavivirus IgG in acute serum WN & SLE PRNT titer approximately equal.Slide7: Virus/Antigen Detection Assays for West Nile Virus mosquito pools avian/equine/other tissues human csf 1. NASBA & TaqMan (0.1 pfu) 2. Virus isolation (1 pfu) Antigen capture ELISA, RT-PCR & VecTest (10 pfu)Slide8: Antigen-capture ELISA VecTest (WN & SLE) 18/30 (60%) TaqMan positive pools positive. Oral Swabs of avian carcasses Crows & blue jays 100% agreement with assay of brain tissues New Developments in Virus/Antigen Detection Assays for West Nile Virus 15 minInstruments for TaqMan Assays: Instruments Evaluated at CDC PE-ABI 7700 – in use BioRad iCycler – in use PE-ABI 7000 MJ Research DNA Engine OPTICAN Available/Not Evaluated at CDC Cepheid SmartCycler Stratagene Mx4000 Corbett Research RotorGene Instruments for TaqMan AssaysTaqMan Multiplex Assays for Arboviruses: TaqMan Multiplex Assays for Arboviruses WN, SLE, and LAC detected in a single reaction. Up to 4 probes could be utilized (i.e. EEE). Instrument capable of multiplexing. WN-FAM probe SLE-CY5 probe LAC-HEX probeDiagnostic & Reference Section TaqMan & NASBA Assays : Diagnostic & Reference Section TaqMan & NASBA Assays You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.
2b lanciotti Esteban Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINTLite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: (To copy code, click on the text box) Embed: URL: Thumbnail: WordPress Embed Customize Embed The presentation is successfully added In Your Favorites. Views: 107 Category: Entertainment License: All Rights Reserved Like it (0) Dislike it (0) Added: October 24, 2007 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Serological and Molecular Assays for West Nile Virus: Serological and Molecular Assays for West Nile Virus Current Techniques & ImprovementsRecommended Tests for WN Virus: Recommended Tests for WN VirusSlide3: Serological Assays for West Nile Virus human serum/csf equine serum avian (chicken) IgM ELISA IgG ELISA POS NEG PRNT with: SLE, WN, DEN STOPSlide4: WN Serological Data IgM ELISA P/N for WN > 2X IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DEN 4-fold rise in PRNT titer to WN from acute to conv. specimen Typical Human WN Case 83% of 2001 WN cases met criteriaSlide5: WN Serological Data from 2001 IgM ELISA P/N for WN < IgM P/N to SLE. PRNT titer to WN 4-fold higher than to SLE or DENSlide6: WN Serological Data from 2001 IgM ELISA P/N for WN > 2X IgM P/N to SLE. 4-fold rise in PRNT titer to WN from acute to conv. Specimen Flavivirus IgG in acute serum WN & SLE PRNT titer approximately equal.Slide7: Virus/Antigen Detection Assays for West Nile Virus mosquito pools avian/equine/other tissues human csf 1. NASBA & TaqMan (0.1 pfu) 2. Virus isolation (1 pfu) Antigen capture ELISA, RT-PCR & VecTest (10 pfu)Slide8: Antigen-capture ELISA VecTest (WN & SLE) 18/30 (60%) TaqMan positive pools positive. Oral Swabs of avian carcasses Crows & blue jays 100% agreement with assay of brain tissues New Developments in Virus/Antigen Detection Assays for West Nile Virus 15 minInstruments for TaqMan Assays: Instruments Evaluated at CDC PE-ABI 7700 – in use BioRad iCycler – in use PE-ABI 7000 MJ Research DNA Engine OPTICAN Available/Not Evaluated at CDC Cepheid SmartCycler Stratagene Mx4000 Corbett Research RotorGene Instruments for TaqMan AssaysTaqMan Multiplex Assays for Arboviruses: TaqMan Multiplex Assays for Arboviruses WN, SLE, and LAC detected in a single reaction. Up to 4 probes could be utilized (i.e. EEE). Instrument capable of multiplexing. WN-FAM probe SLE-CY5 probe LAC-HEX probeDiagnostic & Reference Section TaqMan & NASBA Assays : Diagnostic & Reference Section TaqMan & NASBA Assays