BlastomycosisinDogs

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Blastomycosis in Dogs: 

Blastomycosis in Dogs L. Joseph Wheat MiraVista Diagnostics, and Alfred M. Legendre University of Tennessee Dept of Veterinary Medicine

Epidemiology: 

Epidemiology Proximity to water Outdoor activities Beaver dams Small outbreaks “Microfoci”

Fungal Stain : 

Fungal Stain

Clinical Findings & Involved Tissue: 

Clinical Findings & Involved Tissue Clinical findings Fever 62% Respiratory 49% Skin lesions 49% Depression 48% Anorexia 44% Ocular 43% Weight-loss 37% CNS 6% Involved tissues Lung 85% Lymph nodes 65% Eye 41% Skin 26% Bone 24% Cutaneous 13% Testes/GU 11% Brain 4%

Radiographic Findings: 

Radiographic Findings Radiographic findings Nodulo interstit 39% Lymphadenop 29% Interstitial 22% Alveolar 9% Mass 9% Pleural 8% Normal 6% N=115, LSU

Cutaneous and Bone Lesions: 

Cutaneous and Bone Lesions

Other Common Manifestations: 

Other Common Manifestations

Sensitivity of Cytology: 

Sensitivity of Cytology Tissue Node or abscess 52% Eye 7% Tracheal wash 6% Biopsy 9% Bone, node, sub-cutaneous

Antigen Detection: 

Antigen Detection

Antigen and Serology: 

Antigen and Serology Antigen % pos Urine 94 Serum 87 Antibody 76

Antigen and Antibody Clearance : 

Antigen and Antibody Clearance

Antigenuria During Relapse : 

Antigenuria During Relapse

Sensitivity for Diagnosis: 

Sensitivity for Diagnosis Cytology 71-94% Antigen 94% Culture 10-20% Serology 41-83%

Approach to Early Diagnosis: 

Approach to Early Diagnosis Tracheal washing Aspirate/biopsy lesions Antigen-urine, tracheal aspirates, other fluids Serology

Outcome of Treatment : 

Outcome of Treatment

Improvement in Management: 

Improvement in Management Hightened awareness Early diagnosis Antigen, cytology, & serology AmB and Itra for severe disease Itra drug level monitoring Treatment more 4-6 months Monitoring antigen until negative Suppressive therapy if relapse

Summary: 

Summary Early diagnosis important Histopathology Urinary antigen Amphotericin B and itraconazole Initially for severe cases Itraconazole treatment of choice Determine blood level Four to six months and until antigenuria clears Suppressive therapy if relapse