Slide 2:
September 03 2009 T. Lindeman University of Kentucky Insulin-like Growth Factor 1 Receptor
Propagates mitosis
tyrosine kinase receptor and is a transmembrane protein
Assists with growth for tissues and cardiac and skeletal muscles
Pathways are known to be associated with embryonic limb development IGF-1R are anti-apoptotic SO –when investigating cancers – IGF -1R receptors are high !!
It is insinuated and many papers show that they HELP cancers resist the cytotoxic properties most chemo meds and radiation therapy!
Slide 3:
September 03 2009 T. Lindeman University of Kentucky That means that the oxygen molecules that are left over have nowhere to go, cannot be removed and just dominate they roam the cell like rebels! A condition in which antioxidant levels are lower than normal. They can initiate cell death prematurely and have been identified as a leading factor to aging coupled with the mitochondria. No substantial theory yet .. Rebels
ROS
FREE ionized oxygen!! Oxidative Stress
Slide 4:
September 03 2009 T. Lindeman University of Kentucky Cre-lox strategy
to delete the
essential exon 3 PCR
IGF-1R +/+
IGF-1R +/-
IGF-1R -/- Homozygous IGF-1R -/- did not survive past embryonic stage Growth ?
Stunted?
Dwarfism? It is observed that in nematodes that decreased circulation of IGF-1 impairs growth and development BUT.. INCREASES LIFESPAN
In this experiment as IGF-1R -/- were not observed since none survived the embryonic stage.
No observation of dwarfism, heterozygous IGF-1R+/- even though IGF-1R was halved.
Slide 5:
September 03 2009 T. Lindeman University of Kentucky Oxidative Stress
Slide 6:
September 03 2009 T. Lindeman University of Kentucky What is an ANTIGEN???
Slide 7:
September 03 2009 T. Lindeman University of Kentucky Determining if sex is a factor in reference to oxidative stress
Substantiating sex as a parameter of glucose response in igf-1R +/+ , igf-1R+/- Things that can possibly be examined further. Implicated endocrine pathways can be identified and isolated that assist or determine glucose response in conjunction with igf-1R Examining feedback mechanisms relative to the igf-1R Establishing components that protect against Oxidative Stress Since there is already established research that being resistant to oxidative stress promotes longevity then..