4 18

IVOS & CEROSComputerAssistedSpermAnalysis: 

IVOS & CEROS Computer Assisted Sperm Analysis Spermatology 2006

What is CASA?: 

What is CASA? An automated system designed to: Visualize and digitize successive images of sperm Process and analyze the images Provide precise and meaningful information on sperm kinematics and counts Combination of hardware and software Hardware: optics, illuminator, digitizer, computer Software: modules for motility, morphology, and viability analysis

2 Current CASA Models from HT: 

2 Current CASA Models from HT IVOS Sperm Analyzer Built-in optical system Automated, heated stage Fastest analysis: 6 fields in 6 seconds CEROS Sperm Analyzer Complete system Includes negative phase contrast microscope

NEW!! Modular CEROS: 

NEW!! Modular CEROS Choose only the options you need Cost-effective Use your own negative or positive phase contrast microscope Same proven and trusted analysis algorithms

Multi-species Compatibility: 

Multi-species Compatibility Human Non-human primates Domestic / companion animals Production animals Endangered species Laboratory rodents Sea mammals and fish

Customized CEROS for Fish Sperm: 

Customized CEROS for Fish Sperm “Cooled” stage for optimum maintenance of fish sperm motility Applications include: Monitoring sperm motility at hatcheries Testing extension media and spermiation stimulant Monitoring environmental pollutants Reproductive toxicology Fish, sea urchin, etc.

Negative Phase Contrast - Standard: 

Negative Phase Contrast - Standard 10x Negative Phase 20x Negative Phase Provides fastest analysis Provides superb visualization

Getting Ready for Analysis (IVOS): 

Getting Ready for Analysis (IVOS) Load sample in fixed or known depth chamber (e.g. Leja Chamber, Microcell, Makler) Place filled chamber on stage Select multiple analysis fields Press Start Scan

Sperm Analysis: 

Sperm Analysis Captures multiple “snapshots” extremely quickly (e.g., 30 images in 0.5 second). Tracks the motile cells from snapshot to snapshot Static sperm are identified based on motile cell means (size, contrast, intensity) Computer calculates results

Results: 

Results Motility Progressive Motility Concentrations Counts Velocity - 3 types Motion parameters

Playback for Quality Control: 

Playback for Quality Control Motile sperm marked with green track Progressive sperm marked with blue track Slow sperm marked with purple track Static sperm marked with red dot

Motile Playback Sample: 

Motile Playback Sample

Research Options: 

Research Options Obtain data on individual tracks (Edit Tracks) Study sub-populations of sperm (Sort)

Data Management: 

Data Management Clinical Filing Custom designed reports Store reports to disk HDATA ASCII Export Export data in database / spreadsheet compatible format Summary and individual track data

Digital Image Storage: 

Digital Image Storage Ability to save all fields analyzed Recall and reanalyze fields Sequential field analysis – all fields from original analysis automatically analyzed in order Share images with other IVOS/CEROS users Create QC images for training purposes

Morphology Options (Automated): 

Morphology Options (Automated) Dimensions Morphology Human sperm only Analysis based on Dr. Kruger’s Strict Criteria Diff-Quik and PAP stains Metrix Morphology Multi-species Analysis based on user’s definition of normal Diff-Quik, PAP and other high contrast stains

Real-Time Morphology (non-automated): 

Real-Time Morphology (non-automated) Subjective (visual classification) Fast – “REAL-TIME” Use non-stained, immobilized sperm, or standard stained smear Results combined with motility results Morph-merge option

IDENT Fluorescence (IVOS Only): 

IDENT Fluorescence (IVOS Only) Static and motile sperm identification for high-detritus samples Sperm stained with Hoechst 33342 stain All sperm fluoresce under UV light Accurate counts to within 1%

VIADENT Fluorescence (IVOS Only): 

VIADENT Fluorescence (IVOS Only) Sperm stained with Hoechst 33258 stain Only NON-VIABLE sperm fluoresce under UV illumination Counting Motile/Total counts under standard illumination Viable count under fluorescent illumination Only system to perform motility and viability analysis on the same, live sample

Benefits of CASA: 

Benefits of CASA Objectivity: Removes technician bias Accuracy: Best method for determining percent motility, percent progressive motility, and velocity parameters Precision: Repeatable results Sensitivity: Detects even the slightest changes in sperm motion Reliability: Does not get “tired eyes” – can analyze samples all day without sacrificing accuracy

Hamilton Thorne Contact Information: 

Hamilton Thorne Contact Information Phone: 978-921-2050, 800-323-0503 Fax: 978-921-0250 E-mail: info@hamiltonthorne.com Web: www.hamiltonthorne.com 100 Cummings Center, Suite 465E Beverly, MA 01915 USA