Yeast Expression System

Views:
 
     
 

Presentation Description

Yeast Expression System

Comments

Presentation Transcript

PowerPoint 演示文稿:

Yeast Expression System — Creative BioMart

PowerPoint 演示文稿:

As a well-defined and economical eukaryotic expression system for both secretory and intracellular expression, yeast protein expression system is perfectly suitable for large-scale manufacture of recombinant proteins. Creative BioMart has developed yeast expression vectors that are optimized for the efficient production of heterologous proteins. We have also a few strong signal sequences to ensure the highest secretion level.

PowerPoint 演示文稿:

All of below advantages make yeast expression system an ideal host for producing large quantities of properly folded proteins in a short period of time: High yield High productivity Genetics can be easily manipulated Chemically defined media Complex post-translational modifications Stable production strains Durability Ease of scaling up Low protein production cost

PowerPoint 演示文稿:

At Creative BioMart, our expertise in protein expression extends to both Saccharomyces. cerevisiae and Pichia. pastoris expression systems. Expression of recombinant proteins in S. cerevisiae can be done using three types of vectors:

PowerPoint 演示文稿:

Yeast integrating plasmid Replicating single copy plasmid For integration into genomic locus

PowerPoint 演示文稿:

Yeast centromeric plasmid Replicating single copy plasmid For complementation studies

PowerPoint 演示文稿:

Yeast episomal plasmid Replicating multi-copy plasmid For recombinant protein production, multicopy suppression studies, etc.

PowerPoint 演示文稿:

Saccharomyces. cerevisiae: Full genomic sequence known Well developed genetic methods About 6000 genes A model to other eukaryotes GRAS No toxins Ability to secrete proteins Capacity for post-translational modifications

PowerPoint 演示文稿:

Besides, methylotrophic Pichia.pastoris is a distinguished system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per liter of culture. If you're looking for protein production via fed-batch pichia processes, we have the experienced and credentialed staff to move your project forward.

PowerPoint 演示文稿:

Thanks for watching! Contact Creative BioMart Address: 45-1 Ramsey Road Shirley, NY 11967, USA Email: contact@creative-biomart.com Website: http://www.creativebiomart.net

authorStream Live Help