pg 2001

The zNose™ : A New Electronic Nose Technology for Analytical Quality Control Applications: 

The zNose™ : A New Electronic Nose Technology for Analytical Quality Control Applications Why Electronic Noses Speed, Simplicity, Portability, Precision, Accuracy Quality Control of Odor and Flavor Introduction - GC/SAW, A New Technology A New Analytical Tools : the zNoseTM Applications involving Odors and Flavor Dr. Edward J. Staples, Managing Director Electronic Sensor Technology, Newbury Park, California ESTCAL.COM zNOSE.COM September 26, 2001 Presented at Proctor & Gamble Symposia Session: Fast Analysis of Odors and Flavors

Why Electronic Noses: 

Why Electronic Noses Attributes of an Electronic Nose Speed, e.g. 10 seconds, time is $$$ Accuracy e.g. utilize chemical standards according to existing EPA/FDA methods Precision e.g. STDEV <2% and 30 day long term stability Simplicity and Ease of Use What can an Electronic Nose do? Principal component analysis (PCA) of aromatic products e.g measure chemical composition in 10 seconds Trace Analysis, e.g. search for ppb/ppt level “impurities” in an ingredient or final product. Olfactory images e.g. high resolution (500 pixel) two-dimensional images showing hydrocarbon chemistry What kinds of problems does it solve? Needle in the haystack e.g. diacetyl in OJ, TCA in corks Needle without the haystack e.g. purity of water Recipe Control - on-line flavor monitoring Sensory Panels Linkage between Quality Control Engineers and Sensory Panels Biofeedback tool for sensory panels based upon product chemistry

Two New Analytical Tools for QC of Aroma and Flavor : 

Two New Analytical Tools for QC of Aroma and Flavor The zNose is designed to increase speed, productivity, precision, and accuracy in testing of aromatic products. Four ways to improve product quality: 1. Principal Chemical Components 2. Olfactory Image Analysis 3. Trace Analysis of Impurities 4. Sensory Panel Biofeedback The zNose™ - A GC/SAW Electronic Nose with internal preconcentrator and helium gas supply 10 Second Analysis Speed Volatile Compounds - ppb MDL Semi-Volatile Compounds - ppt MDL

A New Electronic Nose Technology.. Fast Gas Chromatography and Surface Acoustic Wave Detectors: 

A New Electronic Nose Technology.. Fast Gas Chromatography and Surface Acoustic Wave Detectors Hydrocarbon Spectrum Analyzer C4-C30 10 Second Analysis Time Integrated Programmable Preconcentrator Electronically Variable Sensitivity Universal Selectivity (non-polar) ppb sensitivity C4-C12 ppt sensitivity >C12 <1 minute Cycle Time User Friendly Windows® Software Long Term Stability Low Cost and Solid State

zNoseSimplified System Diagram: 

zNose Simplified System Diagram

The zNose: A Hydrocarbon Spectrum Analyzer: 

The zNose: A Hydrocarbon Spectrum Analyzer Isothermal Column Conditions Logarithmic Alkane peak separation Maximum Resolving Power Linear Temperature Program e.g. 10oC/second Quasi-Linear Alkane peak separation Maximum Hydrocarbon Span Method 10 Seconds Alkanes

Virtual Chemical Sensors and Olfactory Images: 

Virtual Chemical Sensors and Olfactory Images c6 c10 c14 c20 Radial Spectrum

Indexing Odor Chemistry to Alkane standards: 

Indexing Odor Chemistry to Alkane standards MTG 10 µl (geosmin=20 µg/mL, TCA=20 µg/mL, MIBK= 5 µL/mL) Container: 800 ml tedlar bag Method: 30 sec sample 20° detector : Ramp Column 40° -150°, 12°/sec, Vapor Pressures MIBK: 12 ppm TCA: 28 ppb Geosmin: 33 ppb

Testing Air or Water Samples using 12ps-2a Method : MTG 10 µl spike in 800 mL tedlar bag: 

Testing Air or Water Samples using 12ps-2a Method : MTG 10 µl spike in 800 mL tedlar bag 10 sec sample 20°detector Water sample 10 sec 20 deg detector Vapor Pressures MIBK: 1.68 ppm TCA: 28 ppb Geosmin: 33 ppb

Calibration Methods : 

Calibration Methods 20o detector 5 sec sample 5ps-2a1b 1 µL of stock (100:1) in 40 mL vial 1 µL of stock (100:1) in tedlar bag (800 mL) 20o detector 5 sec sample 5ps-2a1b 3.433 ppm 31.25 ng 68.7 ppm 249.5 ng

Comparing Fragrance Concentration Lifetimes: 

Comparing Fragrance Concentration Lifetimes Time Virtual Chemical Sensor Array

Aroma and Flavor of Spirits and Alcoholic Beverages: 

Aroma and Flavor of Spirits and Alcoholic Beverages zNoses equipped with DB-624 Columns, tenax preconcentrators, and SAW detector was used for study Hydrocarbon Analysis range: C6-C16 Samples were tested in 40 mL vials with Septum lid(20 mL sample volume) Samples tested at RT Measurement Cycle Time: 90 seconds 10o/sec ramp, 40 - 140oC Model 4100 zNose Temperature Controlled Vial Holder

Principal Chemical Component Analyses - “The Recipe”: 

Principal Chemical Component Analyses - “The Recipe” Standards Unknowns

“Needle in Haystack” or Trace Analysis: 

“Needle in Haystack” or Trace Analysis

Chocolate Aroma/Flavor Chemistry: 

Chocolate Aroma/Flavor Chemistry

Quantifying Perfume Concentration in Deodorant: 

Quantifying Perfume Concentration in Deodorant

Must de Caritereau de toilette: 

Must de Cariter eau de toilette

Guerlain PerfumesVaporPrintsTM: 

Guerlain Perfumes VaporPrintsTM

VaporPrintsTMCommon Perfumes: 

VaporPrintsTM Common Perfumes

ShampooNeutragena : 

Shampoo Neutragena

zNoseTM Analysis of soup aroma and flavor: 

zNoseTM Analysis of soup aroma and flavor 12 types of Soup Aroma Analyzed

Campbell’s Soup VaporPrintsTM: 

Campbell’s Soup VaporPrintsTM

Vegetable Soup: 

Vegetable Soup

Product Taintingfrom Plastic Containers : 

Product Tainting from Plastic Containers

Summary of Advantages for Aromatic Product Manufacturers: 

Summary of Advantages for Aromatic Product Manufacturers The zNose is fast, able to speciate and measure the chemical composition of collected vapors in 10 seconds Easy to Learn and Use Windows® Software Time is Money ! The zNose can perform over 300 measurements in an 8 hour shift The zNose is sensitive, parts per billion and even parts per trillion for certain aromatic compounds The zNose provides 2-dimensional high resolution olfactory images based upon product chemistry Principal Component analysis, virtual chemical sensors Trace analysis can easily spot tainting from product packaging as well as detect “counterfeit” products. New biofeedback tool and documentation for sensory panels For more information or to download this paper be sure to visit our web sites :