zetasizer

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ZETASIZER:

ZETASIZER Presented by: BHARAT LAL M.Pharm . ISF COLLEGE OF PHARMACY

HISTORICAL DEVELOPMENTS :

HISTORICAL DEVELOPMENTS 1940s-Wallace H. Coulter developed a technology for counting and sizing particles. 1970-World's first laser-based particle size analyzer. 1997-Systems for in-process particle size measurement were introduced. 2003- Rheology measurements along with the particle. 2006-Chemical imaging was introduced for better understanding

PowerPoint Presentation:

Size determination P.D.I Poly dispersive index Zeta potential Molecular weight PURPOSE

Principle Of zetasizer :

Principle Of zetasizer SPECIFIC light scattering SPECIFIC conductance deviation

SPECIFIC conductance deviation:

SPECIFIC conductance deviation The change in electric resistance due to passages of particles through the aperture is determined which are digitized into digital signals like the pulse height. The recorded pulse height is converted to particle size using the calibration constant. The particle size distribution and counting are the cumulative result of all pulses measured.

SPECIFIC light scattering :

SPECIFIC light scattering Lamp by which light transmitted from the sample fluid and scattered by the particle present in the fluid and detected by the photodetector

Dynamic Light Scattering is used to measure particle size and molecule size. This technique measures the diffusion of particles moving under Brownian motion, and converts this to size and a size distribution using the Stokes-Einstein relationship. It is used to determine the size distribution profile of small particles in suspension or polymers in solution . It can also be used to probe the behavior of complex fluids such as concentrated polymer solutions.:

Dynamic Light Scattering is used to measure particle size and molecule size. This technique measures the diffusion of particles moving under Brownian motion , and converts this to size and a size distribution using the Stokes-Einstein relationship. It is used to determine the size distribution profile of small particles in suspension or polymers in solution . It can also be used to probe the behavior of complex fluids such as concentrated polymer solutions.

Dynamic light scattering:

Dynamic light scattering

Static Light Scattering is used to determine the molecular weight of proteins and polymers. In Static Light Scattering a beam of polarized light is focused onto a particle and the scattered light is detected with a photodiode detector. In Static Light Scattering the intensity of this scattered light is measured, which is proportional to the molar mass and the concentration of the particles in solution. In Static Light Scattering the accessible molar mass range is 103 Da to 109 Da and the particles size range covered is 10 nm to 500 nm. Because in Static Light Scattering the intensity of the scattered light is directly proportional to the product of molar mass (Mw) and sample concentration (c) the following formula can be used to describe the phenomena: ILS ~Mw*c ILS = Light scattering intensity; Mw = Molar mass weight-average; c = Sample concentration :

Static Light Scattering is used to determine the molecular weight of proteins and polymers. In Static Light Scattering a beam of polarized light is focused onto a particle and the scattered light is detected with a photodiode detector . In Static Light Scattering the intensity of this scattered light is measured, which is proportional to the molar mass and the concentration of the particles in solution. In Static Light Scattering the accessible molar mass range is 10 3 Da to 10 9 Da and the particles size range covered is 10 nm to 500 nm . Because in Static Light Scattering the intensity of the scattered light is directly proportional to the product of molar mass (Mw) and sample concentration (c) the following formula can be used to describe the phenomena: I LS ~ M w *c I LS = Light scattering intensity; M w = Molar mass weight-average; c = Sample concentration

Zeta potential:

Zeta potential It is the difference in potential between the surface of tightly bound layer and electroneutral region of the solution.

Significance of zeta potential:

Significance of zeta potential significance of zeta potential is that its value can be related to the stability of colloidal dispersions (e.g. a multivitamin syrup). ZETA POTENTIAL (mV) Stability behavior of the colloid from 0 to ±5 Rapid coagulation or flocculation from ±10 to ±30 Incipient instability from ±30 to ±40 Moderate stability from ±40 to ±60 Good stability more than ±61 Excellent stability

PowerPoint Presentation:

Laser Doppler Micro-electrophoresis is the technique used to measure zeta potential . An electric field is applied to a solution of molecules or a dispersion of particles, which will then move with a velocity related to their zeta potential. This velocity is measured using a patented laser interferometric technique called M3-PALS (Phase analysis Light Scattering). This enables the calculation of electrophoretic mobility, and from this the zeta potential for the accurate measurement of a wide range of sample types and dispersion media including high salt concentrations.

Marketed zetasizer :

Marketed zetasizer The Malvern Zetasizer Zetasizer Nano ZS Zetasizer Nano Z Zetasizer µV

Brookhaven zeta potential analyser:

Brookhaven zeta potential analyser

References :

References Sinko . P. J, Martins Physical pharmacy and Pharmaceutical science , Wolters kluwer publication ,sixth edition, page no. 452-454 . www.malvern.com www.azonano.com www.iesmat.com www.brookhaveninstruments.com www.en.wikipedia.org/wiki/ www.beckmancoulter.com

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