Resealed Erythrocytes: In-vitro characterisation and Novel systems

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RESEALED ERYTHROCYTES: IN-VITRO CHARACTERISATION AND NOVEL SYSTEMS:

RESEALED ERYTHROCYTES: IN-VITRO CHARACTERISATION AND NOVEL SYSTEMS M. Anitha Sri pharmacy2331@gmail.com

In-vitro Characterisation:

In-vitro Characterisation Drug Content In-vitro drug and Hb release Osmotic Fragility Osmotic Shock Turbulence Shock Morphology % Cell Recovery Entrapped Magnetite ESR ZSR

Drug Content::

Drug Content:

Retaining the entrapped magnetite::

Retaining the entrapped magnetite: Magnet placed adjacent to the base of centrifuge tube.

In-vitro Drug Content:

In-vitro Drug Content

Incubation of loaded normocytes at 37 0C in PBS (pH 7.4) at 50% haematocrit in a metabolic rotating wheel incubator. :

Incubation of loaded normocytes at 37 0 C in PBS (pH 7.4) at 50% haematocrit in a metabolic rotating wheel incubator. Metabolic Shaker Incubator

0.8 µ spectropore membrane filter is fitted to a hypodermic syringe to withdraw the samples.:

0.8 µ spectropore membrane filter is fitted to a hypodermic syringe to withdraw the samples. Hypodermic syringe with a membrane filter.

In-vitro Hb release::

In-vitro Hb release:

Osmotic Fragility:

Osmotic Fragility

Change of shape due to osmotic imbalance:

Change of shape due to osmotic imbalance Osmotic Fragility

Osmotic Shock:

Osmotic Shock

Turbulence shock:

Turbulence shock

Morphology & % cell recovery:

Morphology & % cell recovery

Magnetite concentration:

Magnetite concentration

ESR & ZSR:

ESR & ZSR

NOVEL APPROACHES:

NOVEL APPROACHES

Erythrosomes::

Erythrosomes: Erythrosomes are specially engineered vesicular systems in which chemically cross-linked human erythrocyte cytoskeletons are used as a support upon which a lipid bilayer (phosphatidyl choline)is coated.

Nanoerythrosomes:

Nanoerythrosome s Depletion of Hb Extrusion / Sonication / Electrical Breakdown

Preparation of Erythrocyte Ghosts:

Preparation of Erythrocyte Ghosts Methods: Hypotonic Osmosis Lysis Method (by DeLoach et. al.) Method by Sprandel et. al.

Hypotonic Osmotic Lysis Method:

Hypotonic Osmotic Lysis Method

Sprandel Method::

Sprandel Method:

Extrusion Method::

Extrusion Method: Advantages of this method: a) yield vesicles of more uniform size. b) quicker and cheaper Extrusion is to be done under nitrogen pressure.

Sonication Method::

Sonication Method:

Electrical Breakdown Method::

Electrical Breakdown Method:

Drug-nE conjugation::

Drug- nE conjugation:

Parameters to be optimised::

Parameters to be optimised :

Biotinylated erythrocytes:

Biotinylated erythrocytes Biotin molecules are attached to the amino groups present on the RBC. Methods: Direct interaction. Using spacer arm.

Direct Interaction:

Direct Interaction

Using Spacer Arm::

Using Spacer Arm:

References::

References: 1. S.P. Vyas, R. K. Khar, Targeted and controlled drug delivery, Novel carrier systems, Pg No. 387 to 416. 2. Advances in controlled and Novel Drug Delivery, N. K. Jain, Pg No. 332 to 360. 3. Controlled and Novel Drug Delivery, N. K. Jain, Pg No. 256 to 291. 4. Progress in controlled and Novel Drug Delivery Systems, N. K. Jain, Pg No. 387 to 416.

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