EPA - Office of Pesticide Programs

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Slide 1:- Microbiology Laboratory - Analytical Chemistry Laboratory - Environmental Chemistry Lab The OPP Laboratories: Environmental Science CenterFort Meade, Maryland Stennis Space Center Bay St. Louis, MS


Slide 2:Responsibilities for Pesticide Regulation in the U.S. U.S. Environmental Protection Agency (EPA) Pesticide data reviews & registration Sets tolerances Approves product labels U.S. Food & Drug Administration (FDA) Regulates tolerances in agricultural crops & foods Annual Total Diet Study U.S. Department of Agriculture (USDA) Regulates tolerances in meat, poultry, eggs & milk Pesticide Data Program (PDP) monitoring in fruits & vegetables Individual States Verify label claims of products Incidence & complaint monitoring Environmental & food monitoring


How ACB fit into the Pesticide Program :How ACB fit into the Pesticide Program


Activities of the Analytical Chemistry Laboratory :Activities of the Analytical Chemistry Laboratory National Pesticide Standard Repository: all registered pesticides & metabolites in US. Product Chemistry Team: A.I., impurities, label claim, formulation,…. Residue Chemistry Team: Tolerance Method Validation (TMV): Registrant Method Review (mostly review) Multi-Residue Method Development (MRM) Carbamates (LC/FL) OPs (GC/pFPD) Triazoles (LC-MS/MS) Neonicotinoids & Macrocyclic Lactones (LC-MS/MS).


Special Projects to support OPP’s mission :Special Projects to support OPP’s mission Proportionality Study (HED) Relationship between pesticide sprayed and residues found Oxidation of OPs during chlorination of water (EFED) Formation of oxons and other oxidation products upon water chlorination Formetanate HCl in peach & nectarine samples (HED) to obtain data on effect of label change Effect of imidacloprid and metabolites on Bees (EFED) Collaboration with University of Maryland in a study to determine the behavioral effects of imidacloprid on bees. Homeland security (Chemical Warfare Agents) (R5) Develop analytical method for the detection of metabolites of CWA as indicators Global Residues Study (IR4) Effect of geographic location on residues of tomatoes


Techniques used in ACB :Techniques used in ACB Extraction & cleaup Cartridge Solid Phase Extraction Dispersive Solid Phase Extraction (QuEChERS) Analytical Instrumentation UPLC-MS/MS HPLC-UV/FL/MS GC/MS GC/ECD/NPD/pFPD ISO 17025 certification is pending