logging in or signing up Elisa introduction.ppt : C & BE : NCP Abhi2master Download Post to : URL : Related Presentations : Share Add to Flag Embed Email Send to Blogs and Networks Add to Channel Uploaded from authorPOINT lite Insert YouTube videos in PowerPont slides with aS Desktop Copy embed code: Embed: Flash iPad Dynamic Copy Does not support media & animations Automatically changes to Flash or non-Flash embed WordPress Embed Customize Embed URL: Copy Thumbnail: Copy The presentation is successfully added In Your Favorites. Views: 611 Category: Education License: All Rights Reserved Like it (0) Dislike it (0) Added: March 03, 2011 This Presentation is Public Favorites: 0 Presentation Description No description available. Comments Posting comment... Premium member Presentation Transcript Slide 1: CONTENTS Antigens. Antibodies Immunoassay Different Types Of Immunoassay Different Immunological Tests Introduction To Elisa INTRODUCTION TO ELISAANTIGEN: ANTIGEN It is an organic compound a protein, polysaccharide (OR) glycolipid. When entered into the body it induced immune responses by production of antibodies The antibodies so produced neutralizes (OR) destroy the antigen By measuring the concentration of specific antibodies present in the individual (OR) the population it is possible to determine the level of susceptibility and resistance to the infection.ANTIBODIES: ANTIBODIES It is a complex globular protein produced by B-Lymphocytes in response to antigen. Every antibody is made up of heavy chain and light chain molecules HEAVY CHAIN: 450 aminoacids LIGHT CHAIN: 215 aminoacidsSlide 4: Depending on aminoacids sequence present on heavy chain of aminoacids the antibodies are classified into Ig A(ALPHA) Ig D(DELTA) Ig E(EPISILON) Ig G(GAMMA) Ig M ( µ) Humans can produce many specific antibodies . This may be active process by healthy host in response to exposure to foreign antigen (OR) antigens transmitted by placenta (OR) maternal milk from mother to offspring (OR) artificially induced by immunization with live attenuated organisms.HOW ANTIBODIES ARE PRODUCED?: HOW ANTIBODIES ARE PRODUCED?IMMUNOASSAY: IMMUNOASSAY Immunoassay are biochemical tests used to detect (OR) quantify specific antibodies and antigens as in the analyte (blood, urine, bodyfluids). Immunoassay has been developed for number of antibodies for identification and detection of antigen and their products. Immunoassay measures the formation of antigen-antibody complex and detect them. The detection can be done by measuring turbidity, light scattering, radioactive labeling, (OR) chemiluminescent molecule.DIFFERENT TYPES OF IMMUNOSSAY: DIFFERENT TYPES OF IMMUNOSSAY Radio Immunoassay. Fluorescent Immunoassay. Chemiluminescent Immunoassay. Enzyme Immunoassay.IMMUNOLOGICAL TESTS : IMMUNOLOGICAL TESTS Agglutination tests : In this reaction between antibody and antigen occurs. Fluorescent antibody test : In this the reaction between a labelled antigen and a particular antibody occurs and can be visualised using U.V spectroscopy. ELISA : In this the reaction between enzyme labelled antibody and antigens occurs.ENZYME LINKED IMMUNOSORBENT ASSAY(ELISA): ENZYME LINKED IMMUNOSORBENT ASSAY(ELISA) In 1971 Peter Perlmann & Eva Engvall at Stockholm university in Sweden & Anton Schuurs, Bauke Van Weemen in Netherlands independently published papers about method to perform ELISA. Before elisa the only method to perform immunoassay is radioimmunoassay. Today fully automated instrument in medical laboratories around the world use immunoassay principle with an enzyme as reported label.APPLICATIONS: APPLICATIONS ELISA in diagnosis of AIDS. Screening donated blood. Measuring hormone level. Used in toxicology. Used in food industry. Identification and detection of selected proteins. Elisa test can also be used to determine the affinity of antibodies to antigens. Used to determine antibody level in faecal content You do not have the permission to view this presentation. In order to view it, please contact the author of the presentation.