4. ELECTROPHORESIS

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ELECTROPHORESIS:

ELECTROPHORESIS Muhammad Tanveer Khan Assistant Professor Faculty of Pharmacy, Lahore Medical and Dental college

CONTENTS:

History Definition Theory Types Gel electrophoresis Difference between chromatography and electrophoresis CONTENTS

HISTORY:

The technique was first developed by Arne Tiselius in 1930s for the study of serum proteins. HISTORY

DEFINITION:

Electrophoresis is a technique for separating molecules on the basis of their charge and size . At an appropriate pH, the distance the molecules move in an electric field depends on both their overall charge and also their size. DEFINITION

THEORY:

When charged molecules are placed in an electric field, they migrate toward either the positive or negative pole according to their charge. For example; Nucleic acids have a consistent negative charge imparted by their phosphate backbone. They migrate toward the anode. THEORY

TYPES:

TYPES

Gel electrophoresis:

Gel electrophoresis Electrophoresis that involves the use of a gelatinous material such as agarose, acrylamide, starch or cellulose acetate as the matrix is called gel electrophoresis. The gel acts as a support medium for the sample .

Steps:

Steps

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Applications: DNA Sequencing Medical Research Protein research/purification Agricultural testing (seed quality, seed variety)

DIFFERENCE BETWEEN CHROMATOGRAPHY AND ELECTROPHORESIS:

CHROMATOGRAPHY ELECTROPHORESIS Laboratory technique used on large scale Investigative technique used on microscopic level Can be used for liquids, solids and gaseous compounds Used for liquids and solids only Partition coefficient of element is used to carry out investigation Electrical properties of element are used to carry out investigations The mixture is applied at the bottom of the stationary phase and the components move up the phase at different rates. Mixture is applied in the middle of the stationary phase and the components move towards the positive or negative electrode depending on their charge DIFFERENCE BETWEEN CHROMATOGRAPHY AND ELECTROPHORESIS

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